Metabolomics

The comprehensive qualitative and quantitative study of all the small molecules in an organism.

Technology to drive breakthrough science

The Buck’s Metabolomics Core has analytical and informatics capabilities for untargeted (comprehensive) and targeted analysis of metabolites and peptides from biofluids (plasma and serum) and tissue extracts from vertebrate and invertebrate animal models.

Along with relative and absolute levels of metabolites, the platform has also developed comprehensive metabolic flux analysis workflows in cell culture, biofluids, and invertebrate disease models, providing a snapshot of the metabolic state of normal and disease states. This has provided novel insights into the metabolic connections that regulate numerous disease states, from metabolic to neurodegenerative diseases.

  • Arvind Ramanathan, PhD . Core Director

    Dr. Ramanathan received his PhD from the Department of Chemistry at New York University. During his PhD studies, he received additional research training at the Biotechnology Center at University of Wisconsin, Madison. He completed his postdoctoral training at Harvard University and the Broad Institute in chemical biology and mass spectrometry. Dr. Ramanathan is the director of metabolomics at the Buck Institute and is an investigator or co-investigator on numerous NIH grants. He received the Hillblom startup grant to start his lab at the Buck Institute and has extensive experience working with biotech companies and medical centers and foundations across the country, including the Maple Syrup Urine Disease Foundation.

    ARamanathan@buckinstitute.org

  • Sonnet Davis, PhD . Postdoctoral Research Fellow

    Dr. Davis received her undergraduate degree in biochemistry from Oakwood University in 2005 and her PhD in chemistry from the University of Maryland, Baltimore County (UMBC) in 2011. At UMBC, her graduate work focused on developing high-resolution mass-spectrometry-based approaches for structural and functional studies of HIV-1 RNA. Her current work at the Buck Institute lies at the intersection of metabolomics and molecular biology, which provides a unique and emerging platform to better understand disease mechanisms, discover biomarkers of disease, and identify novel drug targets in diseases associated with aging.

    SDavis@buckinstitute.org

  1. Agilent 6520 quadrupole time-of-flight (QTOF) mass spectrometer with 1260 UHPLC

The Agilent 6520 QTOF LC/MS/MS system delivers a combination of mass accuracy, mass resolution, and sensitivity perfect for untargeted metabolite profiling of biological samples.

Agilent 6520 Q-TOF features:

  • Attomole sensitivity
  • ≤2-ppm MS mass accuracy
  • 5-ppm MS/MS mass accuracy
  • Mass resolution to 20,000
  • Ionization modes – electrospray ionization (ESI)
  • Broad mass range from m/z 25 to 20,000
  1. Sciex 4000 QTRAP mass spectrometer with Shimadzu prominence UFLC

The Sciex 4000 QTRAP LC/MS/MS is a hybrid triple quadrupole/linear ion trap system ideal for targeted metabolite profiling of biological samples.

Sciex 4000 QTRAP features:

  • High sensitivity
  • Full-scan MS, MS/MS, and MS3, multiple reaction monitoring (MRM), precursor ion (PI), and neutral loss (NL) scan types
  • Unit mass resolution
  • Ionization modes – electrospray ionization (ESI)
  • Mass range up to 2,000 Da
  1. Varian 2100T GC/MS ion trap mass spectrometer

Varian 2100 T GC/MS/MS features:

  • High sensitivity
  • MS modes (full-scan, selected ion storage [SIS], MS/MS)
  • Ionization modes – electron ionization (EI)
  • Accurate library searches with commercial databases: NIST, Wiley, and PMW
  • Mass range: 10 to 650 u

Technological innovation in the service of scientific advancement

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