The Verdin lab studies the relationship between aging and the immune system. Aging is associated with defects in the adaptive immune system and with a state of chronic activation of the innate immune system (chronic inflammation).
We study how immune aging is regulated by nutrition. We have demonstrated how changes in the relative abundance of key cellular metabolites such as NAD+, acetylcoenzyme A, and the ketone body beta-hydroxybutyrate fluctuate under different nutritional conditions (obesity, calorie restriction, fasting, time-restricted feeding, ketogenic diet) and how this influences immune responses. We are working on key enzymes regulated by these metabolites. These include sirtuins (NAD+), histone acetyltransferases (acetylcoenzyme A), and histone deacetylases (HDACs).
We utilize worm, mice, and human model systems (including stem cells and iPSCs) to define how nutrients influence immune responses during aging. We are also studying how HIV infection induces a state of chronic inflammation and immune activation and how this induces accelerated aging in patients infected with HIV.
Why it matters
We believe chronic inflammation represents a key unifying factor underpinning the development of the chronic diseases of aging, including neurodegeneration (Parkinson’s and Alzheimer’s), cancer, type 2 diabetes, and atherosclerosis (heart attack and stroke). We are intrigued by possible connections between senescence, leaky gut, and innate immune activation. A better understanding of the mechanisms leading to the chronic inflammation associated with aging should provide novel therapeutic targets and potential interventions against human aging.
Science is showing that while chronological aging is inevitable, biological aging is malleable. There's a part of it that you can fight, and we are getting closer and closer to winning that fight.
Eric Verdin, MD
Dr. Verdin is the president and chief executive officer of the Buck Institute for Research on Aging. A native of Belgium, Dr. Verdin received his Doctorate of Medicine (MD) from the University of Liege and completed additional clinical and research training at Harvard Medical School. He has held faculty positions at the University of Brussels, the National Institutes of Health (NIH), and the Picower Institute for Medical Research. Dr. Verdin is also a professor of medicine at University of California, San Francisco.
Dr. Verdin studies how metabolism, diet, and small molecules regulate the activity of HDACs and sirtuins, and thereby the aging process and its associated diseases, including Alzheimer’s. He has published more than 210 scientific papers and holds more than 15 patents. He is a highly cited scientist (top 1 percent) and has been recognized for his research with a Glenn Award for Research in Biological Mechanisms of Aging and a senior scholarship from the Ellison Medical Foundation. He is an elected member of several scientific organizations, including the American Association for the Advancement of Science, the American Society for Clinical Investigation, and the Association of American Physicians. He also serves on the advisory council of National Institute on Drug Abuse at the National Institutes of Health.
Dr. Verdin has extensive experience working with biotech companies. He is a founder of Acylin (purchased by Abbvie). He served on the scientific advisory boards of Elixir, Sirtris (purchased by GSK), Calico (Google), and Nokia, and he also served as advisor to Sofinnova Ventures. Dr. Verdin has also worked for several years as a consultant to Novartis, GSK, J&J, Altana, Roche, Pfizer, and other biotech companies.
Cristina Alexandru PhD Candidate, USC-Buck Biology of Aging Program
Cristina grew up in Romania. She earned her BSc in genetics from the University of Manchester, UK. Her thesis focused on the discovery of novel gene mutations causing Gorlin syndrome, a hereditary cancer syndrome. During her studies she completed a one year internship at the University of Nevada, Reno where she examined protein-protein interactions and protein phosphorylation involved in the regulation of stomach smooth muscle contractility. She joined the Verdin lab in June 2020.
Prasanna Ashok Kumaar, PhD Scientist
Dr. Ashok Kumaar graduated as a Topper with Masters in Biochemistry & Biotechnology from Annamalai University, India. She secured a competitive 5-year National Research Fellowship for pursing her PhD at Anna University, India. Her research focused on developing analytical methods and engineering yeast strains for production of therapeutic proteins and enzymes. Before joining the Buck, she worked as a Research Fellow at Washington State University developing mass spectrometry methods to quantify small molecule drugs and studying interactions between DNA repair and chaperone proteins. Her interest in mass spectrometry approaches to study metabolomic and proteomic changes during aging brought her to the Buck in 2020.
Olga Bielska, PhD Postdoctoral Research Fellow
Olga obtained her BSc and MSc degrees in Biochemistry from the Taras Shevchenko National University of Kyiv in Ukraine. Then she moved to Germany to study Regenerative Biomedicine at the DFG-Center for Regenerative Therapies Dresden (CRTD). She conducted her Master thesis work in the US at the University of Florida and worked there for a year after her graduation from TU Dresden. In Florida, she mastered laser capture microdissection technique (LCMT) and established a staining protocol suitable for RNA isolation from human pancreatic islets of patients with type 1 diabetes for microarray analysis. She moved back to Europe to pursue her PhD degree in the Iargest research unit of France - Institute of Genetics and Molecular and Cellular Biology (IGBMC) affiliated with the University of Strasbourg. There she studied the signaling pathways that regulate mitochondrial dynamics during mitotic progression. Her passion to mitochondrial biology brought her to the lab of Eric Verdin. In the Buck, she studies how mitochondrial metabolism and function affect aging process in humans and mice. She likes gardening, photography, NBA games and interior design.
Rachid Boutoual, PhD Postdoctoral Research Scholar
Dr. Boutoual grew up in Agadir, Morocco. He joined the Verdin lab in October, 2018, following his PhD in molecular and cellular biology from the University of Abdelmalek Essaadi, faculty of Sciences, Tetouan, Morocco. Rachid completed the majority of his studies for his Ph.D. work in the laboratory of Dr. Maria Eugenia Armengod at Principe Felipe Research Center, Valencia, Spain. His doctoral dissertation was focused on the understanding the molecular mechanisms of mitochondrial diseases associated with defects in the post-transcriptional modification of mitochondrial tRNAs catalyzed by the proteins TRMU, MTO1 and GTPBP3. Rachid is currently interested in the role of mitochondrial sirtuins, protein acylation and epigenetic regulation in aging. If time permits, he enjoys playing sports, especially football and swimming, long walks on the beach and of course as he is new to San Francisco, he enjoys exploring this city.
Marlene Cervantes, PhD Postdoctoral Research Fellow
Marlene completed her PhD thesis in the laboratory of the late Dr. Paolo Sassone-Corsi at UC Irvine studying circadian transcription, metabolism and epigenetics. Since joining Dr. Eric Verdin’s lab as a postdoctoral fellow, her research interests have included aging, immunology, and investigating the metabolic consequences of HIV infection and substance abuse. When not in the lab, she enjoys jogging at the beach, visiting different coffee shops, and admiring the scenic views that the Bay Area has to offer.
Jingqi Fang, PhD Postdoctoral Research Fellow
Dr. Fang received his Ph.D. degree in cell biology from the Institute of Biophysics(IBP), Chinese Academic of Sciences(CAS). Currently a postdoctoral research scholar in the Brand lab, he focuses on the investigation of the molecular mechanisms of suppressors of mitochondrial reactive oxygen species production. Before joined the Brand lab, he was studying the mechanism of how the endoplasmic reticulum proteins regulate the aging and senescence process.
Alessia Grozio, PhD Scientist
Alessia earned her PhD in biochemistry at the University of Genoa in Italy where she studied the role of Sirtuin 6 in pancreatic ductal cancer and the extracellular generations of NAD+ precursors by ecto-enzymes in different cancer cell lines. In 2013, she moved to Washington University School of Medicine in St.Louis, where she worked on the characterization of a novel nicotinamide mononucleotide (NMN) transporter. In November 2019, Alessia joined Dr. Verdin lab to focus on the cellular and molecular mechanisms of aging. Outside the lab, Alessia enjoys traveling and shopping at flea markets.
Lauren Haky Dominican University Graduate Student
Lauren earned her bachelor’s degree in biology from Willamette University. During her time there, she participated in a collaborative large-scale mutagenetic screen using Drosophila melanogaster to target the Actin Depolymerizing Factor and cofilin ortholog slingshot. Lauren joined the Buck Institute in 2020 as a research associate in a female reproductive aging lab. In 2021, Lauren joined the Verdin lab as a Dominican University Master’s student. Lauren enjoys painting, hiking with her dog, debating art history theories and is learning to play the ukulele.
Indra Heckenbach PhD candidate, visiting scientist from University of Copenhagen
Indra is pursuing a PhD with the University of Copenhagen. He has an MSc in biophysics and a background in software development. He is focused on applying deep learning and computational methods to aging research and biomedical sciences.
Vicki Hirth Lab Technician
Vicki has spent a career in the health industry working in physical therapy. Although retirement allows her to enjoy life to the fullest, her keen interest in science, healthspan, and wanting to contribute to her community initially brought her to the Buck Institute to volunteer. She also owned her own tennis retail store for 16 years before retiring. She enjoys coming to lab on a part time basis to support research projects through her organizational and cleaning skills. The lab has a good symbiotic relationship with her aptitude.
Herb Kasler, PhD Research Assistant Professor, Flow Cytometry Core Director
Dr. Kasler did his undergraduate work in biology at Duke University and earned his PhD in immunology from University of California, Berkeley, working on the regulation of cell death in T-cell development in the laboratory of Dr. Astar Winoto. Since joining the Verdin lab, his main area of focus has been elucidating the immunobiology of histone deacetylase 7 (HDAC7), an epigenetic regulator with an essential role in the maintenance of immune self-tolerance. With other members of the immunology group, he is working to define the molecular mechanisms whereby the TCR-dependent nuclear export of HDAC7 mediates both negative thymic selection and the differentiation of agonist-selected innate-like T-cell populations. By understanding the important role of HDAC7 in T-cell development, he hopes to gain new insights into the regulation of immune self-tolerance and also to identify novel molecular pathways that can be targeted in autoimmune disease. When he is not pushing back the frontier of human knowledge one miniprep at a time, he likes to cook fabulous food for his friends and family, hike through the many splendid landscapes surrounding the Bay Area, and play at being a suburban farmer.
Ik-Jung Kim, PhD Postdoctoral Research Scholar
Dr. Kim is originally from South Korea. After earning an undergraduate and a master’s degree at Sogang university, he moved to the United States for a PhD training in the field of cellular microbiology. At the University of Illinois at Urbana-Champaign, he worked in Dr. Steven Blanke’s lab to study human infection with Helicobacter pylori, which he found manipulates host metabolism, resulting in a global metabolic shift from a biosynthetic to a catabolic state. As growing older, his deep interest in human metabolism stretched to aging biology and finally drew him to the Buck Institute. Since joining Verdin lab in February, 2019, he strive to understand how the levels of NAD+, a central metabolite involved in energy production and healthspan, are systemically regulated, using in vitro and in vivo models. Outside of the lab, he can be found exploring the beautiful nature in Northern California, or, pampering his hedgehog pets.
Chisaka Kuehnemann Scientist
Chisaka is a PhD candidate in the Buck-USC graduate program, where she is studying cellular senescence, a basic aging process. Prior to joining the Buck, she did oncology drug discovery at Human Genome Sciences. She received a bachelor’s degree from the University of Maryland Baltimore County and a master’s degree from Johns Hopkins University. Her particular research focus is in understanding how certain drugs, such as those used in anti-retroviral therapies, might promote aging by inducing cellular senescence, especially since these drugs have been shown to accelerate aging in human patients. Outside of the lab, she reads science fiction, designs costumes, and is an avid cook.
Ryan Kwok Research Associate
Ryan grew up in Hong Kong. He earned his bachelor’s degree in Biology from Reed College where he wrote his thesis on the effects of the social environment on cichlid telomeres. Ryan joined the Verdin lab in 2019. He assists with various assays as well as maintaining the lab and mouse colony.
Yong-Ho Lee Visiting Scientist
Anne Marker Dominican University Graduate Student
Anne grew up in Frederick, Maryland and moved to California in 2016 to attend Saint Mary’s college of CA. She graduated with a BS in biochemistry in the spring of 2020. Following graduation, Anne joined the Verdin lab as a Dominican University Master’s student. Her research focuses on developing a new mouse model for accelerated aging based on dysfunctional mitochondria.
Hiroyuki Matsui, PhD Postdoctoral Research Fellow
Dr. Matsui joined the Verdin lab in July, 2020. During his PhD, he performed transcriptome analysis of HIV-1 latently-infected cells at the Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University in Japan. He will continue studying the molecular mechanism establishing and maintaining HIV latency. Furthermore, he is interested in the relationship between HIV infection and accelerated aging phenotype of HIV-infected patients. Outside of the lab, he enjoys driving his car and playing with his kids.
Jazmin Morales Research Associate
Jazmin received her Bachelor of Science degree in Molecular Biology from Sonoma State University in 2018. During her time there she studied the significance of B cell survival in Non- Hodgkin’s Lymphoma. She used EMSA to study the interactions of B-Cell activating factor (BAFF) and its exclusive relationships to the maturation and survival of B Cells. In the Verdin lab her work is focused on measuring the replicative lifespan of Saccharomyces Cerevisiae via micro dissection.
Rosalba Perrone, PhD Lead Scientist
Dr. Perrone joined the Verdin lab in January 2017. She earned her PhD in biomedicine at the University of Padua in Italy, studying innovative therapeutic targets at the HIV proviral level. In the Verdin lab, her main focus is to study the role of NAD+ metabolism and NAD+ consuming enzymes in aging and age-associated diseases. Rosalba is part of Napa Therapeutics, a spinout from the Buck Institute based in the Verdin Lab and financed by Juvenescence ltd. Napa Therapeutics is a discovery-stage company focused on NAD+ metabolism with the main goal of developing strategies to increase bioavailable NAD+ and improve healthspan in aging and age-related diseases. Science is not Rosalba’s only big passion. She loves to express herself through creative arts, from dancing to crochet. She loves everything that comes from her home country, Italy - especially the good food and music. Her first goal in life is to always be passionate, curious, and happy. Her motto is: “Resolve to be the sun!”
Rebeccah Riley Senior Research Associate, Verdin Lab Manager
Rebeccah received her bachelor’s degree in molecular and cellular biology from UC Santa Cruz. Early on she began studying the mechanisms of the human papilloma virus using rodent models to understand the correlation between 17B-estradiol and cervical cancer at UCSF. She joined the Buck Institute in 2003 to continue research on novel cancer cell therapies and the role of stem cell maintenance in the aging process. Taking a leap more into management, she recently joined the Verdin lab in 2019 as the lab manager, providing support and expertise to a variety of personnel and projects. In her spare time she trains dogs for search and rescue, passionately trail rides her horses wherever and whenever she can, and enjoys raising two small boys in lovely Sonoma County.
Please contact Rebeccah with any lab management requests for reagents, plasmids, mouse lines, or protocols.
Daria Timonina PhD Candidate, USC-Buck Biology of Aging Program
Daria received her undergraduate degree in biochemistry from the University of Arizona. Her thesis focused on G-Quadruplexes, a naturally occurring secondary structure in DNA. After graduating, she worked as a laboratory technician at Massachusetts General Hospital, using patient samples to study potential lung cancer treatments. In her free time she enjoys snowboarding, climbing, and painting.
Ritesh Tiwari, PhD Research Associate
Dr. Tiwari completed his masters in biotechnology at Madurai Kamraj University and earned his PhD in immunology from National institute of Immunology, New Delhi, India in 2014 where he worked on the influence of impaired vesicular transport in Beige B cells response. Since then he has worked on multiple projects involving universities and industries helping them educate, design and analyse experimental data involving multi-color flow cytometry. He joined the Verdin lab in September 2019 and his work includes providing support to a diverse array of projects in the biology of aging, both by sorting cells and by helping with immunophenotyping and other types of analyses involving Flow cytometry as well as working on role of chromatin modifications, particularly histone acetylation, in immune aging and autoimmune disease. In his spare time he enjoys going for hike or bike ride with his fellow buck colleagues.
Ran Zhang, PhD Postdoctoral Research Fellow
Dr. Zhang grew up in Tongling, China, and joined the Verdin lab in September 2017. He holds a bachelor’s degree in clinical medicine and a PhD in biochemistry and molecular biology from the Chinese Academy of Medical Sciences and Peking Union Medical College. He is currently interested in epigenetic regulation of metabolism and aging. Ran enjoys hiking and cooking.
Yini Zhang, PhD Postdoctoral Research Fellow
Dr. Zhang joined the Verdin lab in February, 2020. She earned her PhD in biochemistry and molecular biology at the Institute of Biophysics, Chinese Academy of Sciences, where she studied the details of the interaction between Ero1a and PDI, which are two ER-located proteins responsible for oxidative protein folding, and investigated the suppression of cervical cancer by targeting Ero1a-PDI interaction. She also studied the function and mechanism of S-nitrosalytion, a post-translational modification of Ero1a. In her free time, she enjoys traveling and reading.
Senior Research Associate, Verdin Lab Manager
Please contact Rebeccah with any lab management requests for reagents, plasmids, mouse lines, or protocols.
Phone: 415-209-2000 x2882
- Verdin, E. (2015 Dec 4). NAD⁺ in aging, metabolism, and neurodegeneration. Science, 350(6265), 1208–13. DOI: 10.1126/science.aac4854. Review. PubMed PMID: 26785480.
- Verdin, E., Ott, M. (2015 Apr). 50 years of protein acetylation: From gene regulation to epigenetics, metabolism and beyond. Nat Rev Mol Cell Biol, 16(4), 258–64. DOI: 10.1038/nrm3931. PubMed PMID: 25549891.
- Gut, P., Verdin, E. (2013 Oct 24). The nexus of chromatin regulation and intermediary metabolism. Nature, 502(7472), 489–98. DOI: 10.1038/nature12752. Review. PubMed PMID: 24153302.
- Shimazu, T., Hirschey, M. D., Newman, J., He, W., Shirakawa, K., Le Moan, N., Grueter, C. A., Lim, H., Saunders, L. R., Stevens, R. D., Newgard, C. B., Farese, R. V. Jr, de Cabo, R., Ulrich, S., Akassoglou, K., Verdin, E. (2013 Jan 11). Suppression of oxidative stress by β-hydroxybutyrate, an endogenous histone deacetylase inhibitor. Science, 339(6116), 211–4. DOI: 10.1126/science.1227166. PubMed PMID: 23223453. PubMed Central PMCID: PMC3735349.
- Hirschey, M. D., Shimazu, T., Jing, E., Grueter, C. A., Collins, A. M., Aouizerat, B., Stančáková, A., Goetzman, E., Lam, M. M., Schwer, B., Stevens, R. D., Muehlbauer, M. J., Kakar, S., Bass, N. M., Kuusisto, J., Laakso, M., Alt, F. W., Newgard, C. B., Farese, R. V. Jr, Kahn, C. R., Verdin, E. (2011 Oct 21). SIRT3 deficiency and mitochondrial protein hyperacetylation accelerate the development of the metabolic syndrome. Mol Cell, 44(2), 177–90. DOI: 10.1016/j.molcel.2011.07.019. PubMed PMID: 21856199. PubMed Central PMCID: PMC3563434.
- Hirschey, M. D., Shimazu, T., Goetzman, E., Jing, E., Schwer, B., Lombard, D. B., Grueter, C. A., Harris, C., Biddinger, S., Ilkayeva, O. R., Stevens, R. D., Li ,Y., Saha, A. K., Ruderman, N. B., Bain, J. R., Newgard, C. B., Farese, R. V. Jr, Alt, F. W., Kahn, C. R., Verdin, E. (2010 Mar 4). SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature, 464(7285), 121–5. DOI: 10.1038/nature08778. PubMed PMID: 20203611. PubMed Central PMCID: PMC2841477.