Morphology and imaging

Allowing researchers to examine the form of living organisms and the relationships between their structures.

Technology to drive breakthrough science

The Morphology and Imaging Core is a centralized facility serving the microanatomical, histological, live-cell and in vivo microscopic imaging and bioenergetic assay needs of all researchers and projects at the Buck Institute as well as external users. Core staff provide a range of support, from direct participation in research projects to routine sample preparation for imaging.

The full-service laboratory customizes and optimizes protocols for histology, cytochemistry, immunostaining, in situ hybridization, functional imaging and image analysis to meet individual needs. Experiments are conducted using brightfield, DIC, fluorescence, laser scanning confocal, two-photon or transmission electron microscopy. State-of-the-art software is employed to analyze and quantify multidimensional data sets. We provide training in all services offered.

  • Martin Brand, PhD . Director

    Martin Brand was trained in the UK at the University of Manchester Institute of Science and Technology, where he received his Bachelor of Science, and at the University of Bristol, where he received his PhD. His postdoctoral work was at Johns Hopkins University in Baltimore, Maryland, with Professor A. L. Lehninger. Dr. Brand was a faculty member of the Biochemistry Department at the University of Cambridge, and he was subsequently a group leader at the Medical Research Council in Cambridge. He moved his laboratory to the Buck Institute in 2008. His research focuses on cellular energy transformations, and his lab uses techniques such as fluorescence imaging and extracellular flux analysis.

  • Akos Gerencser, MD, PhD . Assistant Director

    Akos Gerencser received his MD and PhD in neurosciences at Semmelweis University and his MS in biomedical engineering at Budapest University of Technology and Economics in Hungary. His postdoctoral work was at the Burnham Institute for Medical Research in La Jolla, California, and at the Buck Institute. He has worked with Dr. Brand at the Buck Institute since 2008, initially as a staff scientist and since 2012 as an assistant research professor. His interdisciplinary research merges assay technology development (primarily advanced live-cell fluorescence microscopy) for mitochondrial physiology and bioenergetics with application of these technologies to neurodegenerative diseases, stem cell biology, and type 2 diabetes.

  • T. T.
    Taki Te Koi, BS . Core Manager, Senior Research Associate

    Taki Te Koi graduated from Dominican University of California in San Rafael . She joined the Buck Institute in 2012 and has developed specialized technical expertise for the preparation, acquisition, and analysis of images of cells and tissues. Ms. Te Koi also has an extensive background in fluorescence and electron microscopy imaging and works frequently with collaborators to deliver services that cover the full spectrum of morphology and imaging workflows.

  • S. B.
    Stella Breslin, BS . Research Associate II

    Stella Breslin graduated from Cal State East Bay in 2013 and joined the Buck Institute in 2014. She received a certificate in optical microscopy and advanced bioscience microscopy from the Merritt College Microscopy Program in 2010. She oversees and assists with all core responsibilities, including tissue processing and embedding and histological sectioning of frozen and paraffin samples. She is also involved in core imaging projects and shares general project management duties with collaborators.

  • J. F.
    Jose Flores . Research Associate I

    Jose Flores is currently working on his Bachelor of Science in cell and molecular biology at Sonoma State University. He joined the Buck Institute in 2016 and assists with core services such as tissue embedding, sectioning, and histological staining.

  • Pratiksha Dighe, BTech, MA . Senior Research Associate

    Pratiksha Dighe joined the Buck Institute as part of Dr. Martin Brand’s lab in 2015 and is an integral part of the Brand lab team. She has a master’s degree in biological sciences (stem cell concentration) from Sacramento State University and is currently pursuing a certification course in clinical research and management through the UC Berkeley Extension program. She assists with core services involving Seahorse XF respirometry and associated assays.

Laboratory services

Tissue processing, embedding, and sectioning: frozen, paraffin, or floating

Histological stains: H&E, Cresyl Violet, Oil Red O, etc.

Immunohistochemistry or immunocytochemistry with enzyme-substrate development (HRP, AP) or fluorescent secondaries

 

Imaging services

Functional imaging: imaging of calcium and other ion concentrations, fluorescent protein localization and aggregation, organelle motility, apoptosis, FRET, FRAP, mitochondrial and plasma membrane potential, photoactivation/uncaging, etc.

High content imaging: automated microplate-based multidimensional imaging

Conventional microscopy: brightfield, DIC, fluorescence (blue/green/red/infrared)

Laser scanning confocal and two-photon microscopy: immunolocalization, co-localization, fluorescence correlation spectroscopy, functional time course, slice culture, fly organs, intracranial, etc.

Transmission electron microscopy: sample preparation, sectioning, imaging, image analysis

 

Image processing services

Bitplane Imaris: modules include Imaris Core, Measurement Pro, FilamentTracer, co-localization and batch processing for 3D or 4D reconstruction and analysis for fixed or functional imaging data

Image Analyst MKII: multidimensional time-lapse analysis, customized high content analysis, automated image analysis

Histology

Leica embedding center, sliding, and automated rotary microtomes (RM2155)

Leica CM1950 cryostat

Microm HM505EP cryostat

RMC MT7000 ultramicrotome with diatome diamond knives

Leica TP1020 automated tissue processor

Nikon SMU-zoom dissecting microscope

Dual-view Nikon E400 microscope

 

Light microscopy

Zeiss LSM 780 inverted confocal with 405 nm, 442 nm, and standard RGB lasers. Features constant temperature enclosure and CO2 regulation at the stage for functional assays.

Zeiss LSM 7MP upright deep tissue imager with Chameleon Vision II laser (680 nm to 1080 nm). Features constant temperature enclosure and CO2 regulation at the stage for functional assays.

Zeiss LSM 510 inverted confocal with argon (458 nm, 477 nm, 488 nm, and 514 nm), 543 HeNe, and 633 HeNe lasers for fixed or functional imaging. Features constant temperature enclosure and CO2 regulation at the stage.

Nikon Ti PFS inverted microscope with Cascade 512B EMCCD camera, Sutter filter wheels, and xenon light source for rapid functional image acquisition. Features constant temperature enclosure and CO2 regulation at the stage.

Nikon E800 and TE300 upright microscopes with DS-Fi3 CCD camera, DIC optics, and Hg bulb for fixed sample brightfield, DIC, and epifluorescent imaging

Ni-E upright microscope with motorized stage, epifluorescence, DAPI, GFP, Texas Red, and CY5 filters

 

Electron microscopy

Transmission electron microscopy: FEI-Philips Tecnai 12, tilting stage, Gatan Bioscan CCD

 

Respirometry and flux analysis

Agilent Seahorse XFe24: 24-well extracellular flux analyzer

Agilent Seahorse XFe96: 96-well extracellular flux analyzer

 

Other

Perkin Elmer EnSpire Alpha plate reader: luminescence and AlphaLISA/AlphaScreen microplate reader

We charge researchers at an hourly rate for utilizing core services and equipment.

External contracts are evaluated on a per-project basis.

Please contact the core manager for further information.

Taki Te Koi
Core Manager
TTekoi@buckinstitute.org
415- 209-2000 x 6745

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