Mammalian female reproductive function declines dramatically during aging, as reflected by germ cell pool loss, reduced ovarian endocrine function and an inclination to develop uterine disorders. The Jin lab uses mouse and human models to understand and tackle these problems. First, we are pioneering methods to preserve and study young oocytes before aging initiation via tissue engineering in mice, with the goal of eventually extending this work to humans. Second, we recently identified a novel uterine stem cell population using single-cell lineage tracing in mice, and demonstrated that these stem cells are an important source for uterine homeostasis and regeneration. We are now working to reveal how dysfunction of uterine stem cells or their niche during aging leads to gynecological diseases like cancer and endometriosis. Our long-term goal is to develop stem-cell-based therapeutic strategies. In collaboration with other Buck researchers, we also want to apply our knowledge to understand how reproductive aging impacts aging in other organs, like the brain, in females.
Why it matters
Female aging initiates in the reproductive system then spreads to the whole body. This gender specific aging calendar creates severe health challenges to women. Thus, reproductive health and longevity sets the first key step to women’s wellness.
We are driven by a mission to improve women’s reproductive health, longevity and equality to research ovarian and uterine function and aging.
Shiying Jin, PhD
The Jin lab is pleased to acknowledge the generous support of the following major funders:
Dr. Jin is an assistant professor in the Center for Female Reproductive Longevity and Equality within the Buck Institute for Research on Aging. He received his Ph.D. in reproductive physiology from the China Agricultural University in Beijing. He then had postdoctoral training in reproductive, developmental and stem cell biology at Northwestern University in Chicago as well as in the Department of Embryology, Carnegie Institution for Science in Baltimore. Dr. Jin recently identified the first resident uterine stem cell population in mice. He was awarded the Oncofertility Global Scholar Award by the National Institutes of Health to study female fertility preservation.
Ronald Pandoy Research Associate
Ronald received a BS degree in physiology from the University of California, Santa Barbara in 2010. After working for several years, he decided to expand his education and obtained an additional BS degree in biomedical science from La Sierra University in 2016. Upon completing his additional degree, Ronald enrolled at Walla Walla University to pursue a MS degree in biology. There, he completed his thesis in 2019 which focused on protein degradation pathways in the social amoeba Dictyostelium discoideum. Ronald joined the Lei lab in August 2019. In his spare time, Ronald enjoys all manners of sports and games.
Heather Tanner Research Associate and Lab Manager
Heather obtained her BA degree in anthropology from Western Michigan University. She then worked as an archaeologist across the United States moving to York in the United Kingdom to pursue and complete a MA in landscape archaeology. Deciding to change research objectives, Heather moved to Boston, MA in the United States where she conducted her Master thesis work on the placebo effect in Dr. Emmanuel Pothos’ neuroscience laboratory. In Boston, she mastered brain slice and amperometry electrophysiology technique and mammalian cell culture to observe the placebo effect on the rat central nervous system. Her passion for finding drug candidates and her interest in the reproductive system led her to her first benchwork position in Dr. Lei Lei’s laboratory at the University of Michigan, which she followed to the Buck Institute. Here she researches how and why cytokinesis is interrupted during oogenesis with mouse models. She enjoys water sports, running/walking with her dog Daisey, admiring and making art, and archaeology.
Luyi Zheng, PhD Postdoctoral Research Fellow
Luyi received her MBBS degree in preventive medicine from Fujian Medical University in China in 2011, and her PhD in regeneration and tumor science from Shiga University of Medical Science in Japan in 2017. During her PhD, Luyi studied the effect of Dienogest on ovarian function. Following graduation, Luyi continued her research as a postdoc associate in the same department. Luyi joined the Jin lab in October 2019 where she is studying uterine stem cells and uterus aging. Luyi likes hiking and diving.
- Shiying Jin. 2019. Bipotent stem cells support the cyclical regeneration of endometrial epithelium of the murine uterus. Proceedings of the National Academy of Sciences. 116:6848-6857.
- Shiying Jin, David Martinelli, Xiaobin Zheng, Marc Tessier-Lavigne, Chen-Ming Fan. 2015. Gas1 is a receptor for Sonic Hedgehog to repel enteric axons. Proceedings of the National Academy of Sciences. 112:73-80.
- Shiying Jin, Lei Lei, Ariella Shikanov, Lonnie D. Shea, Teresa K. Woodruff. 2010. A novel two-step strategy for in vitro culture of early-stage ovarian follicles in the mouse. Fertility and Sterility. 93:2633-2639.
- Shiying Jin, Lei Lei, Lonnie D Shea, Mary B Zelinski, Richard L Stouffer, Teresa K Woodruff. 2010. Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation. Fertility and Sterility. 93:2627-32.
- Shiying Jin, Meijia Zhang, Lei Lei, Chao Wang, Maoyong Fu, Gang Ning, Guoliang Xia. 2006. Meiosis activating sterol (MAS) regulates FSH-induced meiotic resumption of cumulus cell-enclosed porcine oocytes via PKC pathway. Molecular and Cellular Endocrinology. 249:64-70.
- Chen H, Jin S, Guo J, Kombairaju P, Biswal S, Zirkin BR. 2015. Knockout of the transcription factor Nrf2: Effects on testosterone production by aging mouse Leydig cells. Molecular and Cellular Endocrinology. 409:113-20.
Shiying Jin’s Pubmed link