03/23

STEAM Engine: How to Use RNAi in the Classroom

Event Registration

RNAi is a phenomenon of gene silencing which offers a quick and easy way to determine the function of a gene. We will use the nematode C. elegans to study this Nobel prize-winning technique that was first identified in C.elegans. It is surprisingly a simple experiment to conduct in the classroom. Double-stranded RNA is introduced into the worms, where it is chopped into small single-stranded RNA that binds to the target mRNA, thus preventing the translation of the mRNA into protein, and producing a change in phenotype.

 The workshop will give teachers the chance to get into the lab and do the experiment, as well as provide a lesson plan, time, and materials to bring the activity into the class.

All instructors, including those at the college level, are welcome to attend, though the lab is designed to be implemented in high schools. Space is limited!

Lunch included

March 23, 2019
9:30 am - 1:30 pm
Buck Institute
8001 Redwood Blvd
Novato, California 94945

Agenda

9am-9:30am

Registration (coffee provided)

9:30am-10:30am

Introduction to c. elegans and RNAi

10:30am-11:30am

Lab session I

11:30am-12pm

Lunch (provided)

12pm-12:45pm

Lab Session II

12:45pm-1:30pm

Bringing RNAi into the Classroom

Directions

The Buck Institute is located 25 miles north of the Golden Gate Bridge.

San Francisco Airport/San Francisco/South Bay:

  • Take Highway 101 North across the Golden Gate Bridge
  • Take the last Novato exit — Atherton/San Marin Drive (just north of the DeLong exit)
  • Turn left at the stoplight, and go west over the freeway overpass
  • Get in the right lane, and turn right at the second stoplight onto Redwood Boulevard
  • Go approximately ½ mile, and turn left onto Buck Center Drive
  • At the top of the hill, turn left into the Visitor Parking Lot

Berkeley/Oakland/Oakland International Airport:

  • Take Highway 80 to Highway 580 West, and cross the Richmond/San Rafael Bridge
  • Take 580 until it merges with Highway 101 North in San Rafael
  • Take the last Novato exit — Atherton/San Marin Drive (just north of the DeLong exit)
  • Turn left at the stoplight, and go west over the freeway overpass
  • Get in the right lane, and turn right at the second stoplight onto Redwood Boulevard
  • Go approximately ½ mile, and turn left onto Buck Center Drive
  • At the top of the hill, turn left into the Visitor Parking Lot

From Sonoma County, take Highway 101 South:

  • Take the first Novato exit (Atherton/San Marin Drive)
  • Turn right at the stoplight, and stay in the right lane
  • Take an immediate right onto Redwood Boulevard
  • Go approximately ½ mile, and turn left onto Buck Center Drive
  • At the top of the hill, turn left into the Visitor Parking Lot

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