MONDAY, OCTOBER 5TH.
8:30-9:00: Introduction.
9:00-10:00: L1. David Nicholls: The proton circuit, the central concept of cellular bioenergetics – volts, amps and ohms.
10:00-10:30: Informal breakouts*.
10:30-11:30: L2. David Nicholls: Essential thermodynamics: driving forces.
11:30-12:00: Informal breakouts.
12:00-13:00: L3. Martin Brand: Function and dysfunction: isolated mitochondria part 1.
13:00-14:00: Informal breakouts (lunch).
14:00-15:00: L4. Martin Brand: Function and dysfunction: isolated mitochondria part 2.
15:00-15:30: Informal breakouts.
15:30-17:30: P1. Martin Brand: The Seahorse XF analyzer: isolated mitochondria: problem solving.
TUESDAY, OCTOBER 6TH.
09:00-10:00: L5. David Nicholls: Function and dysfunction: intact cells.
10:00-10:30: Informal breakouts.
10:30-12:00: P2. David Nicholls: The Seahorse XF analyzer: intact cells: problem solving.
12:00-13:00: L6. Martin Brand: reactive oxygen species.
13:00-14:00: Informal breakouts (lunch).
14:00-15:00: L7. Shona Mookerjee: The Seahorse XF analyzer: quantifying glycolytic and oxidative phosphorylation.
15:00-15:30: Informal breakouts.
15:30-17:30: P3. Shona Mookerjee: The Seahorse XF analyzer: quantifying glycolytic and oxidative phosphorylation: problem solving.
WEDNESDAY OCTOBER 7TH.
09:00-10:00: L8. David Nicholls: Applying the proton circuit to a physiological problem: brown fat and non-shivering thermogenesis.
10:00-10:30: Informal breakouts.
10:30-12:15: P4. David Nicholls: Monitoring plasma and mitochondrial potentials in intact cells: problem solving.
12:15-13:15: L9. David Nicholls: Quality control: how to critically evaluate the bioenergetic literature.
13:15-14:15: Martin Brand, Shona Mookerjee, David Nicholls: free-ranging discussion on the state of bioenergetic research.
14:15-16:00: Breakout rooms available for small-group networking amongst participants.
*Informal breakouts: We will try to replicate the networking opportunities of an in-person course. There will be enough breakout rooms to accommodate 4-5 people per room, often containing either David, Martin, Shona, or a member of the Brand lab. At each break you will be randomly pre-assigned to one of the rooms so you can choose to drop in (or not) with a coffee or lunch to chat informally with each other, or with the course leaders. Ideally, you won’t spend the breaks on your emails or twitter (you can do that anytime without attending a course). Over the three days you should get the opportunity to interact with every other participant. On the final day you have the option of holding follow-up deeper conversations or plan new shared projects with any other attendee (just let us know by Wednesday lunchtime who you want to be roomed with).