Human Pluripotent Stem Cell Courses
2012 Course Dates Will Be Announced Soon
What will you learn?
Methodology required for the successful culture and differentiation of human pluripotent stem cells including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). These include but not limited to techniques for:
- Culture of hESC/iPSC on feeders and in feeder-free defined media
- Examining pluripotent markers by immunocytochemistry and RT-PCR
- Reprogramming of somatic cells to iPSCs
- Differentiation into cells of the three germ layers via embryoid bodies (EB) formation
- Directed differentiation into neural stem cells and subsequently differentiation into neurons and glia including dopaminergic neurons.
Who will teach?
Dr. Xianmin Zeng, Associate Professor, Director, CIRM Shared Research Laboratory for Stem Cells and Aging, Buck Institute for Research on Aging.
Dr. Mahendra Rao, Director, National Center for Regenerative Medicine, NIH.
Where and when?
Two 1-week courses will be offered
Where: CIRM Shared Research Laboratory for Stem Cell and Aging, Buck Institute
When: TBD
Is this course for me?
The course is designed for graduate students, postdocs and technicians in the field of stem cell and regenerative medicine.
A maximum of ten participants will be accepted. There is no cost to participate in the course. Registration is closed.



