Human Pluripotent Stem Cell Courses

2014 Course Dates

What will you learn?
Methodology required for the successful culture and differentiation of human pluripotent stem cells including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). These include but not limited to techniques for:

  • Culture of hESC/iPSC on feeders and in feeder-free defined media
  • Examining pluripotent markers by immunocytochemistry and RT-PCR
  • Reprogramming of somatic cells to iPSCs
  • Differentiation into cells of the three germ layers via embryoid bodies (EB) formation
  • Directed differentiation into neural stem cells and subsequently differentiation into neurons and glia including dopaminergic neurons
  • Engineer iPSC by gene targeting

Where and when?
Two 1-week courses will be offered
Where: CIRM Shared Research Laboratory for Stem Cell and Aging, Buck Institute

First Course:
Monday, April 14th through Friday, April 18th
Second Course:
Monday, April 21st through Friday April 25th

Is this course for me?
The course is designed for graduate students, postdocs and technicians in the field of stem cell and regenerative medicine.
A maximum of ten participants will be accepted. There is no cost to participate in the course. 

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