Additional Facilities & Services

At the Buck we also have a number of state-of-the-art resources available for a fee to BIRC tenants, including a leading stem cell facility and vivarium, as well as core services with expertise in bioinformatics, genomics, imaging, proteomics and metabolomics.

World Leading Stem Cell Facility

A company invested in stem cell research could not find a better home than Buck’s Regenerative Medicine Research Center (RMRC). Current tenants include Cellular Dynamics Inc., a leading developer of stem cell technologies and stem cell banking founded in 2004 by Dr. James Thomson, a pioneer in human embryonic stem cell research. The RMRC houses leading stem cell technology and scientific expertize available to BIRC tenants. Vivarium The Buck Institute

Vivarium

is an 11,000 sq.ft. AAALAC-accredited facility for rats and mice. Vivarium staff are skilled in common technical procedures such as handling and restraint, tattooing, blood collection, oral dosing, colony management, injections and euthanasia. Core equipment includes behavioral apparatus, CT scanner, ultrasound & metabolic cages.

Bioinformatics Core

At the Buck we have in-house bioinformatics expertise in analysis of high-throughput data from large datasets such as next-generation sequencing, proteomics and whole genome sequencing. This Core can also build databases, mobile apps and websites on demand. More information on the Bioinformatics Core.

Flow Cytometry Core

The Buck Institute now hosts a full-service flow cytometry core featuring two highly capable BD instruments. If you want to schedule instrument time, training, or a consultation about experimental design, please email @Flowcore (flowcore@buckinstitute.org) and a core staff person will get back to you promptly.  
Here is a brief summary of the resources that are now available:
• A BD FACSAria II cell sorter with four lasers and 14 color channels, enclosed in a biosafety cabinet that will allow sorting of any P2 or P2* biological materials, including primary human cells and cells infected with replication-defective lentiviruses or other P2 pathogens. We can sort into up to 4 tubes simultaneously or into 96-well plates
• A BD LSR II analytical flow cytometer with the same 4-laser optical configuration as the Aria II, which is equipped with a high-throughput sampler allowing for acquisition of up to 400 samples/hour from 96- or 384-well plates
• Both instruments are housed in a fully equipped P2* tissue culture room to allow for processing of samples in the same location where they are sorted.
• We will support multiple licenses for FlowJo data analysis software, and will provide training in its use as needed.

Please consult Our services guide for more details about what our instruments can do, how to view the instrument calendars, and what our services cost. We look forward to helping you get your science done!

Genomics Core

The Buck Genomics Core has considerable experience with genome-wide expression analyses, from microarrays and RNASeq (“NextGen sequencing”) to high-throughput qPCR using the latest technology. A robust analytical workflow facilitates processing of a broad range of species, tissues and cell types, including single cell analyses.

Morphology & Imaging Core

The Morphology and Imaging Core (MIC) services range from hiring of equipment, to training and/or direct participation by Core members. MIC customizes and optimizes protocols for histology, cytochemistry, immunostaining, functional imaging and image analysis methodologies, using brightfield, DIC, fluorescence, laser confocal, or transmission electron microscopy and state-of-the-art software to analyze and quantify data in up to 4D. More information on the Morphology & Imaging Core.

Proteomics Core

The Proteomics Core innovates, develops and implements advanced protein analytical technologies (including quantitative proteomics, posttranslational modifications, protein dynamics and biomarker discovery) to advance basic biology and biomedical research.

Metabolomics Core

Buck’s Metabolomics Core has the capability to measure a diverse array of small molecules and peptides from a variety of model systems, including cultured cells and mouse tissue.

*More information on any core facilities available on request

Share:
Change text size:
smaller

default

bigger