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Equipment

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Histology

  • Leica Embedding Center, Sliding, and Automated Rotary Microtomes

  • Microm HM505EP Cryostat

  • RMC MT7000 Ultramicrotome with Diatome Diamond Knives

  • Nikon SMU-Zoom Dissecting Microscope

  • Dual-view Nikon E400 Micros

Light Microscopy

  • Zeiss LSM 510 with Argon (458nm, 477nm, 488nm and 514nm), 543 HeNe, 633 HeNe and Chameleon Ultra 2-photon lasers (705nm-980nm) for fixed or functional imaging. Equipped with constant temperature enclosure and CO2 regulation at the stage for functional assays.

  • Nikon Ti PFS with Cascade 512B EMCCD Camera, Sutter Filter Wheels and Xenon Light Source for rapid functional imaging acquisition with constant temperature enclosure and CO2 regulation at the stage for functional assays.

  • Nikon E800 and TE300  with DXM1200f CCD camera, DIC optics and Hg bulb for fixed sample brightfield, DIC and epifluorescent imaging

Electron Microscopy

  • Transmission Electron Microscopy - FEI-Philips Tecnai 12, tilting stage, Gatan Bioscan CCD

Image Analysis

  • Bitplane Imaris -  modules include Imaris, FilamentTracer, Colocalization, AutoAligner-for 3D or 4D reconstruction and analysis for fixed or functional imaging data.

  • Huygens Deconvolution

  • Image Analyst - for 2D intensity based analysis of functional imaging data

Respirometry

  • Seahorse Biosciences XF Extracellular Flux Analyzer

 

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